Detection of contaminants in cell cultures, sera and trypsin. The aim of this study was standardization and application of polymerase chain reaction (PCR) for the detection of contaminants in cell cultures, sera and trypsin.
Five PCR protocols were standardized to assess the presence of genetic material from mycoplasma, porcine circovirus 1 (PCV1), bovine leukemia virus (BLV) or bovine viral diarrhea virus (BVDV) in cell culture samples. PCR reactions for the genes GAPDH and beta- actin were used to evaluate the efficiency of nucleic acid extraction.
The PCR protocols were applied to 8. The tests were also used to assess potential contamination in 1. The results showed the occurrence of the following as DNA cell culture contaminants: 3. PCV1, 2. 3. 9% for BVDV RNA and 2. BLV. In fetal calf sera and trypsin samples BVDV RNA and PCV1 DNA was detected. The results demonstrated that cell culture, sera and trypsin used by different laboratories show a high rate of contaminants.
The results highlight the need for monitoring cell cultures and controlling for biological contaminants in laboratories and cell banks working with these materials. Copyright © 2. 01.
The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
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Abstract. The aim of this study was standardization and application of polymerase chain reaction (PCR) for the detection of contaminants in cell cultures, sera and.
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